Pulmonary surfactant-associated protein D (SP-D) is a 43 kDa member of the collectin family of innate immune modulators. Mouse SP-D cDNA encodes a 19 aa signal sequence and a 355 aa mature region with a 25 aa N-terminal linking-region, a 177 aa hydroxyproline and hydroxylysine collagen-like domain, a 46 aa coiled-coil segment, and a 106 aa, C-terminal collectin-like C-type lectin domain . SP-D is found in serum, plasma, broncho-alveolar lavage (BAL) fluid, and amniotic fluid. It also binds SIRP alpha and the calreticulin/CD91 complex on macrophages. SP-D contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. It Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. It may participate in the extracellular reorganization or turnover of pulmonary surfactant. It binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
Product Name:
Mouse SFTPD/SP-D Recombinant Protein (RPES0708)
Product Code:
RPES0708
Size:
10µg
Species:
Mouse
Expressed Host:
Human Cells
Synonyms:
COLEC7, Collectin 7, Lung surfactant protein D, PSPD, Pulmonary surfactant-associated protein D, SFTPD, SPD, SP-D, SP-Dpulmonary surfactant apoprotein, surfactant protein D, surfactant, pulmonary-associated protein D,SFTP4
Accession:
P50404
Sequence:
Ala20-Phe374
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse SP-D is produced by our Mammalian expression system and the target gene encoding Ala20-Phe374 is expressed with a 6His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
36.7 kDa
AP Mol Mass:
42 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM MES, 150mM NaCl, pH 7.4 .
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
