The Recombinant Mouse SIRP-alpha/CD172a Protein is a high-quality recombinant protein designed for murine biological research applications. This protein serves as an essential reagent in mouse model studies, comparative immunology research, and preclinical therapeutic evaluations, enabling scientists to investigate SIRP-alpha/CD172a biology and its relevance to human disease mechanisms through translational research approaches.
This product (SKU: RPCB1646) is produced using HEK293 cells and features a C-hFC tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 63.86 kDa with an observed molecular weight of 90-120 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant Mouse SIRP-alpha/CD172a Protein (RPCB1646), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-Activity:
Measured by its binding ability in a functional ELISA.Immobilized Human CD47 (RPCB1526) at 2 μg/mL (100 μL/well) can bind Mouse CD172a (RPCB1646) with a linear range of 1.2-55.3 ng/mL.
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don′t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
Recombinant Mouse SIRP-alpha/CD172a Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Human CD47 (RPCB1526) at 2 μg/mL (100 μL/well) can bind Mouse CD172a (RPCB1646) with a linear range of 1.2-55.3 ng/mL.
