T cell immunoglobulin and mucin domain-3 (TIM3), also called hepatitis A virus cellular receptor 2 (HAVCR2), is a transmembrane glycoprotein of the TIM family of immune regulating molecules and plays an important role in the Th1-mediated immune response. TIM3 is expressed on the Th1 cells, CD8 T-cells, monocytes, and dendritic cells, but not on Th2 cells. TIM3 expressed by monocytes and dendritic cells facilitates phagocytosis of apoptotic cells and up-regulates cross-presentation of apoptotic cell-associated antigens through interaction with phosphatidylserine. Engagement of TIM3 by its ligand galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity. Stimulation of TIM3 with an agonistic antibody promotes inflammation through the activation of innate immune cells. TIM3 is also regarded as a potential target molecule for immunotherapy. TIM3 and programmed cell death 1 (PD-1) as two important coinhibitory regulators of T cell responses, have been implicated with the T-cell dysfunction or exhaustion associated with chronic HBV infection including HBV-related HCC.
Product Name:
Mouse TIM-3/HAVCR2 Recombinant Protein (RPES0613)
Product Code:
RPES0613
Size:
10µg
Species:
Mouse
Expressed Host:
Human Cells
Synonyms:
Hepatitis A virus cellular receptor 2 homolog, HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, Tim3, Timd3
Accession:
Q8VIM0
Sequence:
Arg20-Arg191
Fusion tag:
C-Fc
Activity:
Immobilized Galectin 9-His(Cat: PKSH032470) at 2µg/ml(100 ?l/well) can bind Mouse TIM-Fc. The ED50 of Mouse TIM-Fc is 4.053ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Mouse TIM-3 is produced by our Mammalian expression system and the target gene encoding Arg20-Arg191 is expressed with a Fc tag at the C-terminus.
Purity:
> 90% as determined by reducing SDS-PAGE.
Mol Mass:
46.3 kDa
AP Mol Mass:
50-80 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
