The Recombinant MPP8 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C531225) against MPP8. Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression. Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2. Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Mouse samples.
This antibody is suitable for WB applications. It has been validated by WB analysis of MEF, Mouse E12 brain, 4T1, F9. The target is localized to the chromosome, nucleus.
Product Name:
Recombinant MPP8 Monoclonal Antibody
SKU:
AGEL6530
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
Mphosph, MPHOSPH8, MPP, MPP8
Reactivity:
Mouse
Immunogen:
Recombinant human MPP8 fragment
Applications:
WB
Clonality:
Recombinant Monoclonal
Clone No:
C531225
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
MPP8
Conjugation:
Unconjugated
Observed MW:
125 kDa
Calculated MW:
110 kDa
Recommended Dilution:
WB 1:500-1000
UniProt:
Q99549
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
