The Recombinant Mucin 5AC Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110661) against Mucin 5AC. The cysteine residues in the Cys-rich subdomain repeats are not involved in disulfide bonding., Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particules that are subsequently removed by the mucocilary system., PTM:C-, O- and N-glycosylated. O-glycosylated on the Thr-/Ser-rich tandem repeats. C-mannosylation in the Cys-rich subdomains may be required for proper folding of these regions and for export from the endoplasmic reticulum during biosynthesis., PTM:Proteolytic cleavage in the C-terminal is initiated early in the secretory pathway and does not involve a serine protease. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of HT-29 and IHC analysis of Human stomach tissue, Rat stomach tissue. The target is localized to the cytoplasm.
Product Name:
Recombinant Mucin 5AC Monoclonal Antibody
SKU:
AGEL5970
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
Apomucin, Apomucin Major Airway Glycoprotein, Gastric mucin, gastric mucin|mucin, gastric mucin|mucin 5, leB, Lewis B blood group antigen, Major airway glycoprotein, MUC, MUC 5, MUC-5AC, MUC5, MUC5A, MUC5AC, mucin, Mucin 5, Mucin 5 Subtype AC, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5 subtypes A and C tracheobronchial/gastric, Mucin 5AC, Mucin 5AC oligomeric mucus/gel forming, Mucin-5 subtype AC, mucin-5AC, oligomeric mucus/gel forming pseudogene, subtypes A and C, TBM, tracheobronchial, Tracheobronchial mucin, Tracheobronchial Mucin (TBM), tracheobronchial/gastric, tracheobronchial/gastric|mucin 5AC|tracheobronchial mucin
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human Mucin 5AC protein
Applications:
WBIHCIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110661
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
Mucin 5AC
Conjugation:
Unconjugated
Observed MW:
586 kDa
Calculated MW:
586 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P98088
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded human stomach tissue using Recombinant Mucin 5AC Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded rat stomach tissue using Recombinant Mucin 5AC Monoclonal Antibody at dilution of 1:200.
Western Blot with Recombinant Mucin 5AC Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: HT-29 cells. Observed-MW:586 kDa Calculated-MW:586 kDa
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