The Recombinant NCAM1/CD56 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C131325) against NCAM1/CD56. In human hematopoiesis, CD56, also known as neural cell adhesion molecule 1 (NCAM1) is highly expressed in natural killer (NK) cells. In addition, NCAM1 expression was found in rare subsets of T and B lymphocytes, dendritic cells, and neural or mesenchymal stem cells. NCAM1 belongs to the immunoglobulin superfamily of adhesion molecules. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, FCM and IP applications. It has been validated by WB analysis of SH-SY5Y, Mouse cerebellum, Rat cerebellum, FCM analysis of SH-SY5Y and IP analysis of SH-SY5Y cells extracts. The target is localized to the membrane, secreted.
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Flow cytometric analysis of human NCAM1/CD56 expression on SH-SY5Y cells. Cells were stained with purified anti-Human NCAM1/CD56, then a Alexa Fluor 488- conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Immunoprecipitation analysis using anti-NCAM1/CD56 Monoclonal Antibody. Western blot was performed from the immunoprecipitate using NCAM1/CD56 Monoclonal Antibody at a dilution of 1:100. Lane 1: 5% Input, Lane 2: NCAM1/CD56 Monoclonal Antibody, Lane 3: Rabbit monoclonal IgG Isotype Observed-MW:140-180 kDa Calculated-MW:95 kDa
Western Blot with NCAM1/CD56 Monoclonal Antibody at dilution of 1:2000. Lane 1: SH-SY5Y, Lane 2: Mouse cerebellum, Lane 3: Rat cerebellum Observed-MW:140-180 kDa Calculated-MW:95 kDa
