The Recombinant NDUFA1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C320402) against NDUFA1. The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB, IHC and IP applications. It has been validated by WB analysis of HeLa. The target is localized to the Mitochondrion inner membrane; Single-pass membrane protein; Matrix side.