The Recombinant NELF-B Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C221687) against NELF-B. NELF-B is an essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNApolymerase II. The NELF complex causes transcriptional pausing and is counteracted by the P-TEFb kinase complex. It may be able toinduce chromatin unfolding. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human and Mouse samples.
This antibody is suitable for WB, IHC, FCM and IP applications. It has been validated by WB analysis of HeLa,?MCF-7, T47D, NIH-3T3, IHC analysis of Human colon, Human colon cancer, Mouse colon, FCM analysis of HeLa and IP analysis of HeLa cells extracts. The target is localized to the nucleus.
Product Name:
Recombinant NELF-B Monoclonal Antibody
SKU:
RMEA1687
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
COBRA, COBRA1, KIAA, KIAA1182, NELF-B, NELFB
Reactivity:
Human, Mouse
Immunogen:
Peptide. This information is proprietary to PTMab.
Applications:
WBIHCFCMIP
Clonality:
Recombinant Monoclonal
Clone No:
C221687
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
NELF-B
Conjugation:
Unconjugated
Observed MW:
70 kDa
Calculated MW:
66 kDa
Recommended Dilution:
WB 1:10000-20000; IHC 1:100; FCM 1:100; IP 1:50-100
UniProt:
Q8WX92
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Flow cytometric analysis of human NELF-B expression on HeLa cells. Cells were stained with purified anti-Human NELF-B, then a Alexa Fluor 488-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Immunohistochemistry of paraffin-embedded Human colon using NELF-B Monoclonal Antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded Human colon cancer using NELF-B Monoclonal Antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded Mouse colon using NELF-B Monoclonal Antibody at dilution of 1:100.
Immunoprecipitation analysis using anti-NELF-B Monoclonal Antibody. Western blot was performed from the immunoprecipitate using NELF-B Monoclonal Antibody at a dilution of 1:100. Lane 1: 5% Input, Lane 2: NELF-B Monoclonal Antibody, Lane 3: Rabbit monoclonal IgG Isotype Observed-MW:70 kDa Calculated-MW:66 kDa
Western Blot with NELF-B Monoclonal Antibody at dilution of 1:20000. Lane 1: HeLa, Lane 2: MCF-7, Lane 3: T47D, Lane 4: NIH-3T3 Observed-MW:70 kDa Calculated-MW:66 kDa
![Anti-NELFE [R02-7E5] Monoclonal Antibody (AGMB02252)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/273541/679593/anti-nelfe-r02-7e5-monoclonal-antibody-agmb02252__50204.1773039392.jpg?c=2 "Anti-NELFE [R02-7E5] Monoclonal Antibody (AGMB02252)")
