The Recombinant NPC2 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110044) against NPC2. This gene encodes a protein containing a lipid recognition domain. The encoded protein may function in regulating the transport of cholesterol through the late endosomal/lysosomal system. Mutations in this gene have been associated with Niemann-Pick disease, type C2 and frontal lobe atrophy. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC-P, ICC/IF and FC applications. It has been validated by WB analysis of HepG2, HeLa, 293T, A549, HT-1080, RAW 264.7, NIH/3T3, Mouse spleen, Mouse kindey, PC-12, C6, Rat spleen, Rat kidney, IHC analysis of Mouse kidney, Rat kidney and IF analysis of U87-MG.
Product Name:
Recombinant NPC2 Monoclonal Antibody
SKU:
RMEA0058
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
EDDM1, Epididymal protein 1, Epididymal secretory protein, Epididymal secretory protein E1, HE1, Human epididymis-specific protein 1, Niemann-Pick disease type C2, Niemann-Pick disease type C2 protein, NPC, NPC intracellular cholesterol transporter 2, NPC2, Tissue specific secretory protein
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunofluorescent analysis of U87-MG cells (green), and DAPI (blue).
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue sections using NPC2 Antibody (RMEA0058) at a dilution of 1:1500 (10× lens). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0) for 20 min prior to IHC staining.
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue sections using NPC2 Antibody (RMEA0058) at a dilution of 1:1500 (10× lens). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0) for 20 min prior to IHC staining.
Western blot analysis of HepG2,HeLa,293T,A549,HT- 1080,RAW 264.7,NIH/3T3,Mouse spleen,Mouse kindey,PC-12,C6,Rat spleen and Rat kidney cell lysates using NPC2 Antibody (RMEA0058) at a dilution of 1:1000. Observed-MW:17 kDa Calculated-MW:17 kDa
