The Recombinant NRAS Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110593) against NRAS. This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of 3T3-L1. The target is localized to the membrane.
Product Name:
Recombinant NRAS Monoclonal Antibody
SKU:
AGEL5907
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
ALPS, ALPS4, AV095280, CMNS, GTPase, GTPase NRas, HRAS, HRAS1, N ras, N ras protein part 4, N-ras, NCMS, Neuroblastoma RAS viral (v ras) oncogene homolog, NRAS, NRAS1, NS6, OTTHUMP00000013879, OTTMUSP00000023521, RASN, Transforming protein N Ras, Transforming protein N-Ras, v ras neuroblastoma RAS viral oncogene homolog
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human NRAS protein
Applications:
WBIHCIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110593
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
NRAS
Conjugation:
Unconjugated
Observed MW:
22 kDa
Calculated MW:
22 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
P01111
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.