The Recombinant Osteopontin Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110520) against Osteopontin. The protein encoded by this gene is involved inThe attachment of osteoclasts toThe mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found inThe cell membrane and may be involved inThe binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of A549. The target is localized to the secreted.
Product Name:
Recombinant Osteopontin Monoclonal Antibody
SKU:
AGEL5834
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
BNSP, Bone sialoprotein, Bone sialoprotein 1, Bone Sialoprotein I, BSP, BSP1, BSPI, Early T-Lymphocyte Activation, Early T-Lymphocyte Activation 1, ETA, ETA 1, ETA-1, Nephropontin, OPN, Osteopontin, PSEC, PSEC0156, secreted phosphoprotein, secreted phosphoprotein 1, SPP, SPP 1, SPP-1, SPP1, Urinary stone protein, Uropontin
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human Osteopontin protein
Applications:
WBIHCIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110520
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
Osteopontin
Conjugation:
Unconjugated
Observed MW:
55, 65 kDa
Calculated MW:
35 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
P10451
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.