The Recombinant p38 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110549) against p38. The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of HeLa, Mouse testis, Mouse liver, Rat testis, Rat liver and IHC analysis of Rat kidney. The target is localized to the cytoplasm, nucleus.
WB 1:1000-5000; IHC 1:50-300; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
Q16539
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemical analysis of paraffin-embedded kidney tissue sections using p38 Antibody (RMEA0558) at a dilution of 1:75 (10× lens). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0) for 20 min prior to IHC staining.
RatWestern blot analysis of HeLa,Mouse testis,Mouse liver,Rat testis and Rat liver cell lysates using p38 Antibody (RMEA0558) at a dilution of 1:1500. Observed-MW:41 kDa Calculated-MW:41 kDa