The Recombinant PAX6 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C210419) against PAX6. This gene encodes a homeobox and paired domain-containing protein that binds DNA and functions as a regulator of transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. This gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human and Mouse samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of HEK293. The target is localized to the nucleus.
IHC 1:200-1000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P26367
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant PAX6 Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: HEK293 cell lysate. Observed-MW:47 kDa Calculated-MW:47 kDa