The Recombinant Perilipin-1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110983) against Perilipin-1. The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of Rat fat. The target is localized to the endoplasmic reticulum.
IHC 1:400-1000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
O60240
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant Perilipin-1 Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: Rat fat cell lysate. Observed-MW:56 kDa Calculated-MW:56 kDa