The Recombinant Phospho-cdc25C (Ser216) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C210149) against cdc25C (Ser216). This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human and Mouse samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of Hela, Hela was treated with Nocodazole (50ng/mL) for 20 hours and IHC analysis of Human lung carcinoma.
A synthetic phosphopeptide corresponding to residues around Ser216 of the Human Phospho-cdc25C
Applications:
WBIHCIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C210149
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
cdc25C (Ser216)
Conjugation:
Unconjugated
Observed MW:
58 kDa
Calculated MW:
53 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:500-2000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
P30307, P48967
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
0.2 µm filtered solution in PBS
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Human lung carcinoma was stained with anti-Cdc25C (Phospho Ser216) Rabbit antibody
Various whole cell lysates were separated by 4-20% SDS- PAGE, and the membrane was blotted with anti-Cdc25C (Phospho Ser216) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: Hela was treated with Nocodazole (50ng/mL) for 20 hours Predicted band size: 53kDa Observed band size: 58kDa
