The Recombinant Phospho-FOXO3A (Ser253) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110635) against FOXO3A (Ser253). This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of HEK293 and IHC analysis of Human pancreas tissue, Mouse pancreas tissue. The target is localized to the cytoplasm.
AF6q, AF6q21, AF6q21 protein, DKFZp781A0677, FKHR2, FKHRL, FKHRL 1, FKHRL1, FKHRL1P, FKHRL1P2, Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1, Forkhead box O3, Forkhead box O3A, Forkhead box protein O3, Forkhead box protein O3A, Forkhead Drosophila homolog of in rhabdomyosarcoma like 1, Forkhead homolog (rhabdomyosarcoma) like 1, Forkhead in rhabdomyosarcoma like 1, Forkhead in rhabdomyosarcoma-like 1, FOX O3A, FOXO2, FOXO3, FOXO3A, MGC12739, MGC31925
Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to residues around (Ser253) of Human Phospho-FOXO3A
Applications:
WBIHCIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110635
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
FOXO3A (Ser253)
Conjugation:
Unconjugated
Observed MW:
97 kDa
Calculated MW:
71 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
O43524
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded human pancreas tissue using Recombinant Phospho-FOXO3A (Ser253) Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded mouse pancreas tissue using Recombinant Phospho-FOXO3A (Ser253) Monoclonal Antibody at dilution of 1:200.
Western Blot with Recombinant Phospho-FOXO3A (Ser253) Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: HEK293 cells. Observed-MW:97 kDa Calculated-MW:71 kDa