The Recombinant Phospho-IκB-α (Ser32) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C170805) against IκB-α (Ser32). This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IF, IP and ELISA applications. It has been validated by WB analysis of K562. The target is localized to the cytoplasm, nucleus, Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
alpha, EDAID, EDAID2, I kappa B alpha, I-kappa-B-alpha, IkappaBalpha, IKB alpha, IkB-alpha, IKBA, IKBalpha, IκB α, MAD, MAD 3, MAD-3, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF kappa B inhibitor alpha, NF-kappa-B inhibitor alpha, NFKBI, NFKBIA, Nuclear factor of kappa light chain gene enhancer in B cells, Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, pIκBα
Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to residues around (Ser32) of Human Phospho-IκB-α
Applications:
WBIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C170805
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
IκB-α (Ser32)
Conjugation:
Unconjugated
Observed MW:
40 kDa
Calculated MW:
36 kDa
Recommended Dilution:
WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
P25963
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant Phospho-IκB-α (Ser32) Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: K562 lysate. Observed-MW:40 kDa Calculated-MW:36 kDa
