The Recombinant Phospho-mTOR (Ser2448) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110476) against mTOR (Ser2448). The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF and ELISA applications. It has been validated by WB analysis of Hela and IHC analysis of Mouse ovary. The target is localized to the cytoplasm.
dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1), FK506 binding protein 12 rapamycin associated protein 1, FK506 binding protein 12 rapamycin associated protein 2, FK506 binding protein 12 rapamycin complex associated protein 1, FK506 binding protein 12-rapamycin associated protein, FK506 binding protein 12-rapamycin associated protein 1, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP rapamycin associated protein, FKBP12 rapamycin complex associated protein, FKBP12-rapamycin complex-associated protein, FKBP12-rapamycin complex-associated protein 1, FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, Mechanistic target of rapamycin, mTOR, OTTHUMP00000001983, RAFT, RAFT1, Rapamycin and FKBP12 target 1, Rapamycin associated protein FRAP2, Rapamycin target protein, Rapamycin target protein 1, RAPT, RAPT1, Serine/threonine-protein kinase mTOR, SKS
Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to residues around (Ser2448) of Human Phospho-mTOR
Applications:
WBIHCIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110476
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
mTOR (Ser2448)
Conjugation:
Unconjugated
Observed MW:
250 kDa
Calculated MW:
289 kDa
Recommended Dilution:
IHC 1:500-2000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P42345
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded mouse ovary using Recombinant Phospho-mTOR (Ser2448) Monoclonal Antibody at dilution of 1:200.
Western Blot with Recombinant Phospho-mTOR (Ser2448) Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: Hela cells. Observed-MW:250 kDa Calculated-MW:289 kDa
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