The Recombinant Phospho-p53 (Ser46) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C210763) against p53 (Ser46). This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human and Mouse samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of HEK293 and IHC analysis of Human breast carcinoma. The target is localized to the nuclear.
Antigen NY-CO, Antigen NY-CO-13, BCC, BCC7, BMFS, BMFS5, Cellular tumor antigen p, Cellular tumor antigen p53, FLJ92943, LFS, LFS1, Mutant tumor protein, Mutant tumor protein 53, P53, p53 tumor suppressor, Phosphoprotein p, Phosphoprotein p53, TP53, Transformation related protein, Transformation related protein 53, TRP, TRP53, Tumor protein, Tumor protein 53, Tumor protein p53, Tumor suppressor p, Tumor suppressor p53
Reactivity:
Human, Mouse
Immunogen:
A synthetic peptide corresponding to residues around (Ser46) of Human Phospho-p53
Applications:
WBIHCIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C210763
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
p53 (Ser46)
Conjugation:
Unconjugated
Observed MW:
53 kDa
Calculated MW:
44 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
P04637
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded human breast carcinoma using Recombinant Phospho-p53 (Ser46) Monoclonal Antibody at dilution of 1:200.
Western Blot with Recombinant Phospho-p53 (Ser46) Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: HEK293. Observed-MW:53 kDa Calculated-MW:44 kDa
