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Recombinant Phospho-STAT1 (Tyr701) Monoclonal Antibody (AGEL8715)

Datasheet MSDS
SKU AGEL8715
Product Type Antibody
Reactivity Human, Mouse, Rat
Applications WB, IF
Host Species Rabbit
Clonality Monoclonal
Clone C173410
Conjugate Unconjugated
$540

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Description

system_update_altDatasheetsystem_update_altMSDS

Recombinant Phospho-STAT1 (Tyr701) Monoclonal Antibody

The Recombinant Phospho-STAT1 (Tyr701) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C173410) against STAT1. Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.

This antibody is suitable for WB and IF applications. It has been validated by analysis of, WB analysis of Hela cells and IF analysis of Rat spleen. The target is localized to the Cytoplasm;Nucleus;Translocated into the nucleus in response to IFN-gamma-induced tyrosine phosphorylation and dimerization.

Product Name:Recombinant Phospho-STAT1 (Tyr701) Monoclonal Antibody
SKU:AGEL8715
Size:50 µL
Antibody Type:Recombinant Monoclonal Antibody
Synonyms:CANDF7, DKFZp686B04100, IMD31A, IMD31B, IMD31C, ISGF 3, ISGF-3, OTTHUMP00000163552, OTTHUMP00000165046, OTTHUMP00000165047, OTTHUMP00000205845, Signal tr, Signal transducer and activator of transcription 1 91kD, Signal transducer and activator of transcription 1 91kDa
Reactivity:Human, Mouse, Rat
Immunogen:A synthetic peptide corresponding to residues around (Tyr701) of Human Phospho-STAT1
Applications:WBIF
Clonality:Recombinant Monoclonal
Clone No:C173410
Host:Rabbit
Isotype:Rabbit IgG
Target:STAT1
Conjugation:Unconjugated
Observed MW:87 kDa
Calculated MW:87 kDa
Recommended Dilution:WB 1:2000-10000; IF 1:200-1000
UniProt:P42224
Purification:Protein A
Concentration:0.2 mg/mL
Buffer:Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol.
Storage:Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Product ImageWestern Blot analysis of Hela cells with Recombinant Phospho-STAT1 (Tyr701) Monoclonal Antibody at dilution of 1:3000. Lane 1: Hela cell, Lane 2: Hela cell was treated with IFNγ(100ng/ml) for 4 hours. Observed-MW:87 kDa Calculated-MW:87 kDa
Product ImageWestern Blot analysis of Hela cells with Recombinant Phospho-STAT1 (Tyr701) Monoclonal Antibody at dilution of 1:3000. Lane 1: Hela cell, Lane 2: Hela cell was treated with IFNγ(100ng/ml) for 4 hours. Observed-MW:87 kDa Calculated-MW:87 kDa
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