The Recombinant Phospho-Vimentin (Ser39) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C131717) against Vimentin (Ser39). Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB applications. It has been validated by WB analysis of HeLa. The target is localized to the cytoplasm, cytoskeleton, nucleus, membrane.
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Phospho-Vimentin (Ser39) Monoclonal Antibody at dilution of 1:1000. Lane 1: (-) HeLa, Lane 2: (+) HeLa + calyculin A (100nM, 30min), Lane 3: (-) NIH/3T3, Lane 4: (+) NIH/3T3 + calyculin A (100nM, 30min), Lane 5: (-) C6, Lane 6: (+) C6 + calyculin A (100nM, 30min) Observed-MW:56 kDa Calculated-MW:54 kDa