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Recombinant Phospho-YAP1 (Ser127) Monoclonal Antibody (RMEA1701)

Datasheet MSDS
SKU RMEA1701
Product Type Antibody
Reactivity Human
Applications WB, IHC, IF, FCM
Host Species Rabbit
Clonality Monoclonal
Clone C311701
Conjugate Unconjugated
€459 - €639

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Description

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Recombinant Phospho-YAP1 (Ser127) Monoclonal Antibody

The Recombinant Phospho-YAP1 (Ser127) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C311701) against YAP1 (Ser127). Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein. Plays a key role to control cell proliferation in response to cell contact. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.

This antibody is suitable for WB, IHC, IF and FCM applications. It has been validated by WB analysis of HeLa, PANC-1, IHC analysis of Human colon cancer, IF analysis of MCF-7 and FCM analysis of PANC-1. The target is localized to the cytoplasm, nucleus.

Product Name:Recombinant Phospho-YAP1 (Ser127) Monoclonal Antibody
SKU:RMEA1701
Size:50 µL, 100 µL
Antibody Type:Recombinant Monoclonal Antibody
Synonyms:65 kDa Yes associated protein, 65 kDa Yes-associated protein, Basic protease inhibitor, BPTI, COB, COB1, Pancreatic Trypsin Inhibitor, Trasylol, YAP, YAp 1, YAP 65, YAP1, YAP2, YAP65, yes -associated protein delta, Yes associated protein 1, Yes associated protein 1 65kDa, Yes associated protein 2, yes associated protein beta, YKI, Yorkie homolog
Reactivity:Human
Immunogen:phosphorylated human YAP1 (Ser1270) peptide
Applications:WBIHCIFFCM
Clonality:Recombinant Monoclonal
Clone No:C311701
Host:Rabbit
Isotype:Rabbit IgG
Target:YAP1 (Ser127)
Conjugation:Unconjugated
Observed MW:70-100 kDa
Calculated MW:54 kDa
Recommended Dilution:WB 1:500-2000; IHC 1:100-200; IF 1:50; FCM 1:50-100
UniProt:P46937
Purification:Protein A
Concentration:1 mg/mL
Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Product ImageFlow cytometric analysis of human Phospho-YAP1 (Ser127) expression on PANC-1 cells. Cells were stained with purified anti-Human Phospho-YAP1 (Ser127), then a Alexa Fluor 488-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Product ImageImmunofluorescent analysis of (4% Paraformaldehyde) fixed MCF-7 cells using anti-Phospho-YAP1 (Ser127) Monoclonal Antibody at dilution of 1:50.
Product ImageImmunohistochemistry of paraffin-embedded Human colon cancer using Phospho-YAP1 (Ser127) Monoclonal Antibody at dilution of 1:200.
Product ImageWestern Blot with Phospho-YAP1 (Ser127) Monoclonal Antibody at dilution of 1:2000. (-): HeLa, (+): HeLa+Okadaic A+ calyculin A (100 nM, 60 min) Observed-MW:70-100 kDa Calculated-MW:54 kDa
Product ImageWestern Blot with Phospho-YAP1 (Ser127) Monoclonal Antibody at dilution of 1:2000. Lane 1: PANC-1 Observed-MW:70-100 kDa Calculated-MW:54 kDa
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