The Recombinant Pim-1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C331646) against Pim-1. Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases. Pim-1, a serine/threonine kinase highly expressed in hematopoietic cells, plays a critical role in the transduction of mitogenic signals and is rapidly induced by a variety of growth factors and cytokines. Pim-1 cooperates with c-Myc in lymphoid cell transformation and protects cells from growth factor withdrawal and genotoxic stress-induced apoptosis. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB applications. It has been validated by WB analysis of K562,?NK-92, TF-1, Raji. The target is localized to the cytoplasm, nucleus, membrane.
Peptide. This information is proprietary to PTMab.
Applications:
WB
Clonality:
Recombinant Monoclonal
Clone No:
C331646
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
Pim-1
Conjugation:
Unconjugated
Observed MW:
33, 34 kDa
Calculated MW:
36 kDa
Recommended Dilution:
WB 1:1000
UniProt:
P11309
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Pim-1 Monoclonal Antibody at dilution of 1:1000. Lane 1: K562, Lane 2: NK-92, Lane 3: TF-1, Lane 4: Raji Observed-MW:33, 34 kDa Calculated-MW:36 kDa
