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Recombinant Pyruvate Dehydrogenase E1 α Monoclonal Antibody (RMEA0638)

Datasheet MSDS
SKU RMEA0638
Product Type Antibody
Reactivity Human, Mouse, Rat
Applications WB, IHC, IF, IP, ELISA
Host Species Rabbit
Clonality Monoclonal
Clone C110631
Conjugate Unconjugated
€409 - €559

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Description

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Recombinant Pyruvate Dehydrogenase E1 α Monoclonal Antibody

The Recombinant Pyruvate Dehydrogenase E1 α Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110631) against Pyruvate Dehydrogenase E1 α. The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.

This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of HEK293 and IHC analysis of Human kidney tissue, Mouse kidney tissue. The target is localized to the mitochondrion matrix.

Product Name:Recombinant Pyruvate Dehydrogenase E1 α Monoclonal Antibody
SKU:RMEA0638
Size:50 µL, 100 µL
Antibody Type:Recombinant Monoclonal Antibody
Synonyms:E1 alpha polypeptide 1, mitochondrial, ODPA, PDH, PDHA, PDHA1, PDHAD, PDHCE1A, PDHE1 A type I, PDHE1-A type I, PDHα, PHE1A, Pyruvate Dehydrogenase (lipoamide) alpha 1, Pyruvate dehydrogenase complex, Pyruvate Dehydrogenase E1 alpha, Pyruvate dehydrogenase E1 component subunit alpha, somatic form
Reactivity:Human, Mouse, Rat
Immunogen:Recombinant Human Pyruvate Dehydrogenase E1 α protein
Applications:WBIHCIFIPELISA
Clonality:Recombinant Monoclonal
Clone No:C110631
Host:Rabbit
Isotype:Rabbit IgG
Target:Pyruvate Dehydrogenase E1 α
Conjugation:Unconjugated
Observed MW:43 kDa
Calculated MW:43 kDa
Recommended Dilution:IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:P08559
Purification:Protein A
Concentration:0.2 mg/mL
Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Product ImageImmunohistochemistry of paraffin-embedded human kidney tissue using Recombinant Pyruvate Dehydrogenase E1 α Monoclonal Antibody at dilution of 1:200.
Product ImageImmunohistochemistry of paraffin-embedded mouse kidney tissue using Recombinant Pyruvate Dehydrogenase E1 α Monoclonal Antibody at dilution of 1:200.
Product ImageWestern Blot with Recombinant Pyruvate Dehydrogenase E1 α Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: HEK293 cells. Observed-MW:43 kDa Calculated-MW:43 kDa
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