The Recombinant Raf-1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C170288) against Raf-1. This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IF and ELISA applications. It has been validated by WB analysis of C6. The target is localized to the membrane, cytoplasm, nucleus.
WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
P04049
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant Raf-1 Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: C6 cell lysate. Observed-MW:73 kDa Calculated-MW:73 kDa
