Natural cytotoxicity triggering receptor 3 ligand 1(B7-H6) is a glycosylated member of the B7 family of immune costimulatory proteins. Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif. Both of the Ig-like domains carry N-linked glycosylation. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells. It does not show binding to NKp44, NKp46, or NKG2D. Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis. B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors.
Rat B7-H6/NCR3LG1 Recombinant Protein (RPES3978)
1.Measured by its binding ability in a functional ELISA. 2.Immobilized rat NCR3-His at 10µg/mL (100?L/well) can bind rat B7-H6-Fc , the EC50 of rat B7-H6 -Fc is 1-20ng/mL.
<1.0 EU per µg protein as determined by the LAL method.
A DNA sequence encoding the rat NCR3LG1 (XP_006223356.1) (Met1-Ser308) was expressed with the Fc region of human IgG1 at the C-terminus.
> 95 % as determined by SDS-PAGE
Lyophilized from sterile PBS, pH 7.4
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.