Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Rat IL-6/Interleukin-6 Recombinant Protein (RPES3914)
Product Code:
RPES3914
Size:
20µg
Species:
Rat
Expressed Host:
E. coli
Synonyms:
IL6
Accession:
NP_036721.1
Sequence:
Phe25-Thr211
Activity:
Measured in a cell proliferation assay using T1165 mouse plasmacytoma cells.The ED50 for this effect is typically 1-6 ng/ml.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the rat IL6 (Phe25-Thr211) was expressed and purified with an initial Met.
Purity:
> 90 % as determined by SDS-PAGE
Mol Mass:
21.9 kDa
AP Mol Mass:
22 kDa
Formulation:
Lyophilized from sterile 40% acetonitrile, 0.1% TFA
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
