Interleukin-17F (IL-17F) is a cytokine that shares sequence similarity with IL17. The most notable role of IL-17 is it involvement in inducing and mediating proinflammatory responses. IL-17 is commonly associated with allergic responses. IL-17F is expressed by activated T cells, and was expressed only in activated CD4+ T cells and activated monocytes. IL-17F has been shown to stimulate the production of several other cytokines, including IL6 and IL8. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. Recombinant human IL-17F did not stimulate the proliferation of hematopoietic progenitors or the migration of mature leukocytes. However, it markedly inhibited the angiogenesis of human endothelial cells and induced endothelial cells to produce IL-2, TGF-{beta}, and monocyte chemoattractant protein-1. IL-17F stimulates the production of other cytokines and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. IL-17F stimulates PBMC and T-cell proliferation. It also function in inhibiting angiogenesis By similarity. IL-17F plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.
Product Name:
Rat Interleukin7F/IL7F Recombinant Protein (RPES3416)
Product Code:
RPES3416
Size:
20µg
Species:
Rat
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
IL17F,IL-17F
Accession:
NP_001015011.1
Sequence:
Met1-Ala153
Fusion tag:
C-His
Activity:
1. Measured by its binding ability in a functional ELISA.2. Immobilized rat IL17F-His at 10 µg/mL (100 ?L/well) can bind Rat IL17RA-Fc3 . The EC50 of Rat IL17RA-Fc3 is 0.28-0.66 µg/mL.
Endotoxin:
<1.0 EU per µg protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the rat Il17f (NP_001015011.1) (Met1-Ala153) was expressed with a polyhistidine tag at the C-terminus.
Purity:
> 90 % as determined by SDS-PAGE
Mol Mass:
16.4 kDa
Formulation:
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
