Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons. References
Product Name:
Rat PCSK9 Recombinant Protein (RPES1578)
Product Code:
RPES1578
Size:
20 µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
NARC-1,Narc1,PC9,Pcsk9
Accession:
NP_954862.2
Sequence:
Met 1-Gln 691
Fusion tag:
C-His
Activity:
1. Measured by its ability to bind biotinylated human LDLR in a functional ELISA.2. Measured by its ability to bind biotinylated mouse LDLR in a functional ELISA.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method
Protein Construction:
A DNA sequence encoding the rat PCSK9 (NP_954862.2) (Met 1-Gln 691) was expressed, fused with a polyhistidine tag at the C-terminus.
Purity:
> 97 % as determined by SDS-PAGE
Mol Mass:
72.8 kDa
AP Mol Mass:
20 kDa and 62 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
