Syndecan-1 also known as SDC1 and CD138, is the most extensively studied member of the syndecan family. It is found mainly in epithelial cells, but its expression is developmentally regulated during embryonic development. Syndecan-1/SDC1/CD138 has been shown to mediate cell adhesion to several ECM molecules, and to act as a coreceptor for fibroblast growth factors, potent angiogenic growth factors involved also in differentiation. Syndecan-1/SDC1/CD138 expression is reduced during malignant transformation of various epithelia, and this loss correlates with the histological differentiation grade of squamous cell carcinomas, lacking from poorly differentiated tumours. In squamous cell carcinomas of the head and neck, positive syndecan-1 expression correlates with a more favourable prognosis. Experimental studies on the role of Syndecan-1 in malignant transformation have shown that Syndecan-1/SDC1/CD138 expression is associated with the maintenance of epithelial morphology, anchorage-dependent growth and inhibition of invasiveness in vitro.
Product Name:
Rat Syndecan/SDC1 Recombinant Protein (RPES4152)
Product Code:
RPES4152
Size:
100 µg
Species:
Rat
Expressed Host:
HEK293 Cells
Synonyms:
SDC1,Synd1,CD138
Accession:
P26260
Sequence:
Met1-Lys253
Fusion tag:
C-Fc
Activity:
1. Measured by its binding ability in a functional ELISA. Immobilized human PTN at 10 µg/ml (100 ?l/well) can bind rat SDC1-Fc, The EC50 of rat SDC1-Fc is 0.35-0.81 µg/ml.2. Measured by its binding ability in a functional ELISA. Immobilized mouse PTN at 10 µg/ml (100 ?l/well) can bind rat SDC1-Fc, The EC50 of rat SDC1-Fc is 0.4-1.1 µg/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method
Protein Construction:
A DNA sequence encoding the rat SDC1 (P26260) (Met1-Lys253) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Purity:
> 90 % as determined by SDS-PAGE
Mol Mass:
51.2 kDa
AP Mol Mass:
92 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
