The Recombinant RPA70 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C311567) against RPA70. RPA70 also named as HSSB, REPA1, RF-A, RP-A, p70, is a component of a heterotrimeric complex, composed of 70, 32/30 and 14 kDa subunits, collectively known as RPA. RPA is a single stranded DNA binding protein, whose DNA binding activity is believed to reside entirely in the 70 kDa subunit. The complex is required for almost all aspects of cellular DNA metabolism such as DNA replication, recombination, cell cycle and DNA damage checkpoints, and all major types of DNA repair including nucleotide excision, base excision, mismatch and double-strand break repairs. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB, IHC-P, ICC/IF, FC and IP applications. It has been validated by WB analysis of HepG2, A549 and IF analysis of HeLa. The target is localized to the nucleus.
Product Name:
Recombinant RPA70 Monoclonal Antibody
SKU:
AGEL6872
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
REPA1, RP-A p70, RPA1
Reactivity:
Human
Immunogen:
A synthetic peptide of human RPA70
Applications:
WBIHC-PICC/IFFCIP
Clonality:
Recombinant Monoclonal
Clone No:
C311567
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
RPA70
Conjugation:
Unconjugated
Observed MW:
70 kDa
Calculated MW:
68 kDa
Recommended Dilution:
WB 1:1000; IHC-P 1:100-200; ICC/IF 1:100-200; IP 1:10-50; FCM 1:50-100
UniProt:
P27694
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.