The Recombinant RUNX3 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C331428) against RUNX3. RUNX3 is a member of the Runt family of transcription factors, playing an important role in the suppression of gastric epithelium cell proliferation, dorsal root ganglia neurogenesis, and T cell differentiation. RUNX3 is also involved in Caspase-3-dependent apoptosis. Protein complexes containing RUNX3 and various transcription factors, such as Smads or β-catenin/TCF4, have tumor suppressor activity and regulate downstream target gene transcription. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB and IP applications. It has been validated by WB analysis of MCF-7(Negative control), Ramos, Raji, HT-1080 and IP analysis of Raji cells extracts. The target is localized to the nucleus, cytoplasm.
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunoprecipitation analysis using anti-RUNX3 Monoclonal Antibody. Western blot was performed from the immunoprecipitate using RUNX3 Monoclonal Antibody at a dilution of 1:50. Lane 1: 10% Input, Lane 2: RUNX3 Monoclonal Antibody, Lane 3: Rabbit monoclonal IgG Isotype Observed-MW:44-46 kDa Calculated-MW:46 kDa
Western Blot with RUNX3 Monoclonal Antibody at dilution of 1:1000. Lane 1: MCF-7(Negative control), Lane 2: Ramos, Lane 3: Raji, Lane 4: HT-1080 Observed-MW:44-46 kDa Calculated-MW:46 kDa
![Anti-RUNX3 [R06-1Z-6] Monoclonal Antibody (AGMB03730)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/275019/677903/anti-runx3-r06-1z-6-monoclonal-antibody-agmb03730__65245.1773034097.jpg?c=2 "Anti-RUNX3 [R06-1Z-6] Monoclonal Antibody (AGMB03730)")
