Mitochondrial import inner membrane translocase subunit TIM16 (TIM16) is an ssential component of the PAM complex. PAM complex is required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, TIM16 is required to regulate activity of mtHSP70 (SSC1) via its interaction with PAM18/TIM14. TIM16 may act by positioning PAM18/TIM14 in juxtaposition to mtHSP70 at the translocon to maximize ATPase stimulation.
Product Name:
S. cerevisiae TIM16 Recombinant Protein (RPES4008)
Recombinant S. cerevisiae TIM16 is produced by our E.coli expression system and the target gene encoding Thr54-Ala119 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
7.9 kDa
AP Mol Mass:
11 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM Tris, 300mM NaCl, pH8.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
