The SARS-CoV-2 Spike S1 Recombinant Protein represents a critical molecular component of the SARS-CoV-2 virus, playing an essential role in viral entry and host cell infection mechanisms. This protein is extensively utilized in COVID-19 research, vaccine development, therapeutic antibody screening, and diagnostic assay development, serving as a fundamental tool for understanding viral pathogenesis and developing countermeasures against coronavirus infections.
This product (SKU: CARP01261) is produced using HEK293 cells and features a C-His&Avi tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 77.67 kDa with an observed molecular weight of 110-120 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
High quality, high purity and low endotoxin recombinant Recombinant SARS-CoV-2 Spike S1 Protein (CARP01261), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Formulation:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.Contact us for customized product form or formulation.
Bio-Activity:
Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Spike S1 at 2 μg/mL (100 μL/well) can bind recombinant Human ACE2 with a linear range of 0.15-6.85 ng/mL.
Immobilized Human ACE2 on COOH Chip can bind SARS-COV-2 Spike S1 with an affinity constant of 24.5 nM as determined in a SPR assay (Nicoya OpenSPR).
Reconstitution:
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Storage:
Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.
Recombinant SARS-CoV-2 Spike S1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of SARS-COV-2 Spike S1 Protein with His and Avi tag (CARP01261) was greater than 95% as determined by SEC-HPLC.
Immobilized Recombinant SARS-CoV-2 Spike S1 at 2μg/mL (100 μL/well) can bind recombinant Human ACE2 with a linear range of 0.15-6.85 ng/mL.
