Recombinant SARS Spike/RBD Protein (RBD, His Tag)

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SKU:
RPES0053
€1,479

Description

Background

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, Oacetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

Key Information Description

Product Name

Recombinant SARS Spike/RBD Protein (RBD, His Tag)

Size

100µg

SKU

RPES0053

Synonyms

coronavirus s1 Protein, SARS; coronavirus s2 Protein, SARS; coronavirus spike Protein, SARS; cov spike Protein, SARS; coronavirus RBD Protein, SARS; RBD Protein, SARS; S1 Protein, SARS; s2 Protein, SARS; Spike RBD Protein, SARS

Species

Virus

Expression host

Baculovirus-Insect Cells

Sequence

Arg306-Phe527

Accession

AAX16192.1

Mol_Mass

26.5 kDa

AP_Mol_Mass

35.1 kDa

Tag

C-His

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per µg as determined by the LAL method.

Storage

Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Formulation

Lyophilized from sterile 20mM PBS,300mM NaCl,10% glycerol,pH 7.0

Reconstitution

Please refer to the printed manual for detailed information.

USAGE

For Research Use Only.

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