The Recombinant SGK1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C331575) against SGK1. Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt. SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human samples.
This antibody is suitable for WB applications. It has been validated by WB analysis of SK-MEL-28, A375, HeLa. The target is localized to the membrane, nucleus, mitochondrion, cytoplasm.
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with SGK1 Monoclonal Antibody at dilution of 1:1000. Lane 1: SK-MEL-28, Lane 2: A375, Lane 3: HeLa Observed-MW:45-55 kDa Calculated-MW:49 kDa