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Recombinant Smad1 Monoclonal Antibody (RMEA0744)

Datasheet MSDS
SKU RMEA0744
Product Type Antibody
Reactivity Human, Mouse, Rat
Applications WB, IF, ELISA
Host Species Rabbit
Clonality Monoclonal
Clone C170741
Conjugate Unconjugated
€409 - €559

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Description

system_update_altDatasheetsystem_update_altMSDS

Recombinant Smad1 Monoclonal Antibody

The Recombinant Smad1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C170741) against Smad1. The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.

This antibody is suitable for WB, IF and ELISA applications. It has been validated by WB analysis of A549. The target is localized to the cytoplasm, nucleus, Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 (PubMed:15647271). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15647271). Exported from the nucleus to the cytoplasm when dephosphorylated (By similarity).

Product Name:Recombinant Smad1 Monoclonal Antibody
SKU:RMEA0744
Size:50 µL, 100 µL
Antibody Type:Recombinant Monoclonal Antibody
Synonyms:BSP, BSP-1, BSP1, hSMAD, hSMAD1, JV4, JV4-1, JV41, MAD Homolog, MAD Homolog 1, Mad-Related Protein, Mad-Related Protein 1, MADH, MADH1, MADR, MADR1, Mothers Against Decapentaplegic Homolog, Mothers Against Decapentaplegic Homolog 1, Mothers Against DPP Homolog, Mothers Against DPP Homolog 1, SMAD 1, SMAD Family Member 1, Smad1, Transforming Growth Factor-Beta-Signaling Protein, Transforming Growth Factor-Beta-Signaling Protein 1
Reactivity:Human, Mouse, Rat
Immunogen:Recombinant Human Smad1 protein
Applications:WBIFELISA
Clonality:Recombinant Monoclonal
Clone No:C170741
Host:Rabbit
Isotype:Rabbit IgG
Target:Smad1
Conjugation:Unconjugated
Observed MW:52 kDa
Calculated MW:52 kDa
Recommended Dilution:WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:Q15797
Purification:Protein A
Concentration:0.2 mg/mL
Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Product ImageWestern Blot with Recombinant Smad1 Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: A549 lysate. Observed-MW:52 kDa Calculated-MW:52 kDa
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