The Recombinant SMC1A Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C231607) against SMC1A. he cohesin complex consists of a heterodimer between SMC1 (SMC1A or B) and SMC3, bound by additional RAD21 and STAG proteins (STAG1, 2, or 3). These proteins form a ring-like structure that mediates the cohesion of two sister chromatids after DNA replication in S phase. RAD21 and STAG2 are phosphorylated by Polo-like kinase (PLK) during prophase, which leads to the dissociation of cohesin complexes from the chromosome arms; however, cohesin remains bound to centromeres until anaphase. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human and Mouse samples.
This antibody is suitable for WB applications. It has been validated by WB analysis of SW480,?HepG2, 4T1, NIH/3T3. The target is localized to the nucleus.
Product Name:
Recombinant SMC1A Monoclonal Antibody
SKU:
AGEL6912
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
CDLS, CDLS2, Chromosome segregation protein SmcB, DXS423E, KIAA, KIAA0178, MGC138332, SB1.8, Segregation of mitotic chromosomes 1, SMC, SMC protein 1A, SMC-1-alpha, SMC-1A, SMC1, SMC1 (structural maintenance of chromosomes 1 yeast) like 1, SMC1 structural maintenance of chromosomes 1 like 1, SMC1A, SMC1alpha, SMC1L, SMC1L1, SMCB, Structural maintenance of chromosomes 1A, Structural maintenance of chromosomes protein 1A
Reactivity:
Human, Mouse
Immunogen:
Peptide. This information is proprietary to PTMab.
Applications:
WB
Clonality:
Recombinant Monoclonal
Clone No:
C231607
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
SMC1A
Conjugation:
Unconjugated
Observed MW:
160 kDa
Calculated MW:
141 kDa
Recommended Dilution:
WB 1:1000-2000
UniProt:
Q14683
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.