The Recombinant SOCS3 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C531624) against SOCS3. The suppressor of cytokine signaling (SOCS) family members are negative regulators of cytokine signal transduction that inhibit the Jak/Stat pathway. The SOCS family consists of at least 8 members including the originally identified cytokine-inducible SH2-containing protein (CIS1), as well as SOCS1-7. Each SOCS family member contains a central SH2 domain and a conserved carboxy-terminal motif designated as the SOCS box. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Mouse samples.
This antibody is suitable for WB applications. It has been validated by WB analysis of RAW264.7,?RAW264.7+LPS(10 ng, ml 48h). The target is localized to the cytoplasmic side of plasma membrane.
Peptide. This information is proprietary to PTMab.
Applications:
WB
Clonality:
Recombinant Monoclonal
Clone No:
C531624
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
SOCS3
Conjugation:
Unconjugated
Observed MW:
25, 15 kDa
Calculated MW:
25 kDa
Recommended Dilution:
WB 1:1000-2000
UniProt:
O14543
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with SOCS3 Monoclonal Antibody at dilution of 1:2000. Lane 1: RAW264.7, Lane 2: RAW264.7+LPS(10 ng, ml 48h) Observed-MW:25, 15 kDa Calculated-MW:25 kDa
