The Recombinant SQSTM1/p62 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C111006) against SQSTM1/p62. This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of 293T, A431, A549, HeLa, HepG2, K562, NIH/3T3, Mouse brain, Rat brain, Rat lung, Rat heart and IHC analysis of Human stomach. The target is localized to the cytoplasm, nuclear.
Product Name:
Recombinant SQSTM1/p62 Monoclonal Antibody
SKU:
RMEA1007
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
A170, DMRV, EBI 3 associated protein of 60 kDa, EBI 3 associated protein p, EBI 3 associated protein p60, EBI3 associated protein of 60 kDa, EBI3 associated protein p60, EBI3-associated protein of 60 kDa, EBIAP, FTDALS, FTDALS3, MGC127197, NADGP, ORCA, OSF, OSF-6, Osi, OSIL, Oxidative stress induced like, p60, p62, p62B, Paget disease of bone 3, PDB, PDB 3, PDB3, Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa, Phosphotyrosine independent ligand for the Lck SH2 domain p62, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, PKC-zeta-interacting protein, Protein kinase C-zeta-interacting protein, Sequestosome 1, sequestosome-1, SQSTM, SQSTM 1, SQSTM1, STAP, STONE, STONE14, Ubiquitin binding protein p62, Ubiquitin-binding protein p62, ZIP, ZIP 3, ZIP3
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human SQSTM1/p62 protein
Applications:
WBIHCIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C111006
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
SQSTM1/p62
Conjugation:
Unconjugated
Observed MW:
60 kDa
Calculated MW:
48 kDa
Recommended Dilution:
IHC 1:200-1000; WB 1:500-5000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
Q13501
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemical analysis of paraffin-embedded Human stomach tissue sections using SQSTM1/p62 Antibody (RMEA1007) at a dilution of 1:300 (10× lens). Heat mediated antigen retrieval was performed with Tris- EDTA buffer (pH 9.0) for 20 min prior to IHC staining.
Western blot analysis of 293T,A431,A549,HeLa,HepG2,K562,NIH/3T3,Mouse brain,Rat brain,Rat lung and Rat heart cell lysates using SQSTM1/p62 Antibody (RMEA1007) at a dilution of 1:750. Observed-MW:60 kDa Calculated-MW:48 kDa
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