Cholera toxin is protein complex secreted by the bacterium Vibrio cholerae. It is responsible for the massive, watery diarrhea characteristic of cholera infection. Cholera enterotoxin subunit B (CTXB) pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.
Product Name:
Vibrio cholerae Toxin B Recombinant Protein (RPES4147)
Product Code:
RPES4147
Size:
10µg
Species:
Vibrio cholerae
Expressed Host:
E.coli
Synonyms:
Cholera enterotoxin subunit B,Cholera enterotoxin B chain,Cholera enterotoxin gamma chain,Choleragenoid,ctxB,toxB
Accession:
P01556
Sequence:
Thr22-Asn124
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Vibriocholerae Toxin B is produced by our E.coli expression system and the target gene encoding Thr22-Asn124 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
11.8 kDa
AP Mol Mass:
11 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 50mM Tris, 200mM NaCl, pH8.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
