The Recombinant Villin Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C121307) against Villin. Epithelial cell-specific Ca2+-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB and IHC applications. It has been validated by WB analysis of HT-29, HepG2, Mouse kidney, Rat kidney and IHC analysis of Human colon, Rat colon. The target is localized to the cytoplasm.
Product Name:
Recombinant Villin Monoclonal Antibody
SKU:
AGEL6612
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
D2S, D2S1471, VIL, VIL1, villin, villin-1
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant human Villin fragment
Applications:
WBIHC
Clonality:
Recombinant Monoclonal
Clone No:
C121307
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
Villin
Conjugation:
Unconjugated
Observed MW:
95 kDa
Calculated MW:
93 kDa
Recommended Dilution:
WB 1:500-2000; IHC 1:200-1000
UniProt:
P09327
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded Human colon using Villin Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat colon using Villin Monoclonal Antibody at dilution of 1:200.
Western Blot with Villin Monoclonal Antibody at dilution of 1:2000. Lane 1: HT-29, Lane 2: HepG2, Lane 3: Mouse kidney, Lane 4: Rat kidney Observed-MW:95 kDa Calculated-MW:93 kDa