Anti-REG3A Antibody (CAB2101)

Antibody Name:	Anti-REG3A Antibody
Antibody SKU:	CAB2101
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 27-175 of human REG3A (NP_002571.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, Raji, HT-29, SW480

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 27-175 of human REG3A (NP_002571.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EEPQ RELP SARI RCPK GSKA YGSH CYAL FLSP KSWT DADL ACQK RPSG NLVS VLSG AEGS FVSS LVKS IGNS YSYV WIGL HDPT QGTE PNGE GWEW SSSD VMNY FAWE RNPS TISS PGHC ASLS RSTA FLRW KDYN CNVR LPYV CKFT D
Gene ID:	5068
Uniprot:	Q06141
Cellular Location:	Secreted
Calculated MW:	19kDa
Observed MW:	15kDa

Synonyms:

REG3A, HIP, HIP/PAP, INGAP, PAP, PAP-H, PAP1, PBCGF, REG-III, REG3

Background:

This gene encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. The mature protein also functions as an antimicrobial protein with antibacterial activity. Alternate splicing results in multiple transcript variants that encode the same protein.

UniProt Protein Function:	REG3A: Might be a stress protein involved in the control of bacterial proliferation.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 2p12 Cellular Component: extracellular space; cytoplasm Molecular Function:carbohydrate binding Biological Process: heterophilic cell adhesion; cell proliferation; multicellular organismal development; negative regulation of keratinocyte differentiation; acute-phase response
NCBI Summary:	This gene encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. The mature protein also functions as an antimicrobial protein with antibacterial activity. Alternate splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Nov 2014]
UniProt Code:	Q06141
NCBI GenInfo Identifier:	464341
NCBI Gene ID:	5068
NCBI Accession:	Q06141.1
UniProt Related Accession:	Q06141
Molecular Weight:	175
NCBI Full Name:	Regenerating islet-derived protein 3-alpha
NCBI Synonym Full Names:	regenerating islet-derived 3 alpha
NCBI Official Symbol:	REG3A
NCBI Official Synonym Symbols:	HIP; PAP; PAP1; REG3; INGAP; PAP-H; PBCGF; HIP/PAP; REG-III
NCBI Protein Information:	regenerating islet-derived protein 3-alpha; REG-3-alpha; reg III-alpha; PAP homologous protein; human proislet peptide; pancreatitis-associated protein 1; proliferation-inducing protein 34; proliferation-inducing protein 42; hepatocarcinoma-intestine-pancreas; pancreatic beta cell growth factor; hepatointestinal pancreatic protein; regenerating islet-derived protein III-alpha
UniProt Protein Name:	Regenerating islet-derived protein 3-alpha
UniProt Synonym Protein Names:	Hepatointestinal pancreatic protein; HIP/PAP; Human proislet peptide; Pancreatitis-associated protein 1; Regenerating islet-derived protein III-alpha; Reg III-alpha
Protein Family:	Regenerating islet-derived protein
UniProt Gene Name:	REG3A
UniProt Entry Name:	REG3A_HUMAN

	Western blot analysis of extracts of various cell lines, using REG3A antibody (CAB2101) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat lung using REG3A antibody (CAB2101) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using REG3A antibody (CAB2101) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse stomach using REG3A antibody (CAB2101) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using REG3A antibody (CAB2101). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-REG3A Antibody (CAB12479)	Human Regenerating Islet Derived Protein 3 Alpha / REG3A ELISA Kit
	Human REG3 alpha (Regenerating Islet Derived Protein 3 Alpha) CLIA Kit (HUES00737)
	Human REG3 alpha (Regenerating Islet Derived Protein 3 Alpha) ELISA Kit (HUES02341)
	Human Regenerating islet-derived protein 3-alpha (REG3A) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)