Anti-Ren1 Antibody (PACO64491)

Antibody Name:	Ren1 Antibody (PACO64491)
Antibody SKU:	PACO64491
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	WB:1:1000-1:5000, IHC:1:100-1:500
Species Reactivity:	Rat
Immunogen:	Recombinant Rat Ren1 protein (65-264AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Antigen Affinity purified & Affinity purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Western Blot. Positive WB detected in: Rat Heart tissue, Rat Liver tissue. All lanes: Ren1 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 45 kDa. Observed band size: 45 kDa.

IHC image of PACO64491 diluted at 1:100 and staining in paraffin-embedded rat kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Background:	Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Synonyms:	Renin (EC 3.4.23.15) (Angiotensinogenase), Ren1, Ren

UniProt Protein Function:	REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.4.23.15; Secreted, signal peptide; Secreted; Protease Cellular Component: extracellular space; membrane; cytoplasm; intracellular Molecular Function:peptidase activity; insulin-like growth factor receptor binding; endopeptidase activity; aspartic-type endopeptidase activity; receptor binding Biological Process: response to drug; response to cAMP; establishment of blood-nerve barrier; hormone-mediated signaling; drinking behavior; renin-angiotensin regulation of aldosterone production; cell maturation; male gonad development; response to lipopolysaccharide; proteolysis; angiotensin maturation; response to organic substance; beta-amyloid metabolic process; regulation of MAPKKK cascade; regulation of blood pressure; renin-angiotensin regulation of blood volume; mesonephros development; kidney development
NCBI Summary:	catalyzes the cleavage of a Leu-Xaa bond in angiotensinogen to generate angiotensin [RGD, Feb 2006]
UniProt Code:	P08424
NCBI GenInfo Identifier:	148747255
NCBI Gene ID:	24715
NCBI Accession:	NP_036774.4
UniProt Secondary Accession:	P08424,Q63497,
UniProt Related Accession:	P08424
Molecular Weight:
NCBI Full Name:	renin
NCBI Synonym Full Names:	renin
NCBI Official Symbol:	Ren
NCBI Official Synonym Symbols:	Ren1; RENAA; RATRENAA
NCBI Protein Information:	renin; angiotensinogenase; renin 1 structural
UniProt Protein Name:	Renin
UniProt Synonym Protein Names:	Angiotensinogenase
Protein Family:	Protein
UniProt Gene Name:	Ren1
UniProt Entry Name:	RENI_RAT

ELISA Kits

Rat REN (Renin) ELISA Kit (RTFI01481)

Rat REN (Renin) CLIA Kit (RTES00023)

Rat Renin (Ren1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)