null

Anti-RHBDD1 Antibody (CAB14350)

SKU:
CAB14350
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
  • Cell Death Antibodies 1 Anti-RHBDD1 Antibody CAB14350
  • Cell Death Antibodies 1 Anti-RHBDD1 Antibody CAB14350
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-RHBDD1 Antibody
Antibody SKU:CAB14350
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 206-315 of human RHBDD1 (NP_115652.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, BxPC-3, Raji, mouse brain, mouse kidney, rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 206-315 of human RHBDD1 (NP_115652.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TQGP LKKI MEAC AGGF SSSV GYPG RQYY FNSS GSSG YQDY YPHG RPDH YEEA PRNY DTYT AGLS EEEQ LERA LQAS LWDR GNTR NSPP PYGF HLSP EEMR RQRL HRFD SQ
Gene ID:84236
Uniprot:Q8TEB9
Cellular Location:Endoplasmic reticulum membrane, Membrane, Mitochondrion, Multi-pass membrane protein
Calculated MW:12kDa/35kDa
Observed MW:35kDa
Synonyms:RHBDD1, RRP4
Background:
UniProt Protein Function:RHBDD1: Intramembrane-cleaving serine protease that cleaves single transmembrane or multi-pass membrane proteins in the hydrophobic plane of the membrane, luminal loops and juxtamembrane regions. Involved in regulated intramembrane proteolysis and the subsequent release of functional polypeptides from their membrane anchors. Functional component of endoplasmic reticulum-associated degradation (ERAD) for misfolded membrane proteins. Required for the degradation process of some specific misfolded endoplasmic reticulum (ER) luminal proteins. Participates in the transfer of misfolded proteins from the ER to the cytosol, where they are destroyed by the proteasome in a ubiquitin-dependent manner. Functions in BIK, MPZ, PKD1, PTCRA, RHO, STEAP3 and TRAC processing. Involved in the regulation of exosomal secretion; inhibits the TSAP6-mediated secretion pathway. Involved in the regulation of apoptosis; modulates BIK-mediated apoptotic activity. Also plays a role in the regulation of spermatogenesis; inhibits apoptotic activity in spermatogonia. Belongs to the peptidase S54 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.4.21.105; Membrane protein, integral; Membrane protein, multi-pass; Protease

Chromosomal Location of Human Ortholog: 2q36.3

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane

Biological Process: membrane protein intracellular domain proteolysis; negative regulation of apoptosis; post-translational protein modification; spermatid differentiation

UniProt Code:Q8TEB9
NCBI GenInfo Identifier:83405869
NCBI Gene ID:84236
NCBI Accession:
UniProt Secondary Accession:Q8TEB9,Q495B9, Q53S43, Q5EBM8, Q6P5V8, Q8IV60, Q9H057
UniProt Related Accession:Q8TEB9
Molecular Weight:12,346 Da
NCBI Full Name:Rhomboid domain containing 1
NCBI Synonym Full Names:rhomboid domain containing 1
NCBI Official Symbol:RHBDD1  
NCBI Official Synonym Symbols:RRP4  
NCBI Protein Information:rhomboid-related protein 4
UniProt Protein Name:Rhomboid-related protein 4
UniProt Synonym Protein Names:Rhomboid domain-containing protein 1; Rhomboid-like protein 4
UniProt Gene Name:RHBDD1  
UniProt Entry Name:RHBL4_HUMAN
Anti-RHBDD1 Antibody (CAB14350)Western blot analysis of extracts of various cell lines, using RHBDD1 antibody (CAB14350) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-RHBDD1 Antibody (CAB14350)Immunofluorescence analysis of L929 cells using RHBDD1 Polyclonal Antibody (CAB14350) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Related Products