B7 Homolog 4 (B7-H4) is glycosylated member of the B7 family of immune costimulatory proteins.It is widely expressed, including in kidney, liver, lung, pancreas, placenta, prostate, spleen, testis and thymus. B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. It also involved in promoting epithelial cell transformation.
Product Name:
Rhesus macaque B7-H4/VTCN1 Recombinant Protein (RPES3986)
Product Code:
RPES3986
Size:
10µg
Species:
Rhesus macaque
Expressed Host:
Human Cells
Synonyms:
B7S1, B7x, Vtcn1, B7h.5, B7-H4, B7H4T-cell costimulatory molecule B7x, B7S1VCTN1, B7XPRO1291, FLJ22418, Immune costimulatory protein B7-H4, Protein B7S1, T cell costimulatory molecule B7x, V-set domain containing T cell activation inhibitor 1, V-set domain-containing T-cell activation inhibitor 1
Accession:
F7B770
Sequence:
Phe29-Ala258
Fusion tag:
C-His
Activity:
Immobilized Human GFRA1-His(Cat: PKSH033670) at 0.5µg/ml(100 ?l/well) can bind Human GDNF. The ED50 of GDNF is 14.73ug/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Rhesus macaque B7-H4 is produced by our Mammalian expression system and the target gene encoding Phe29-Ala258 is expressed with a 6His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
26.4 kDa
AP Mol Mass:
40-60 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
