Western blot analysis of various lysates, using Rig-I/DDX58 antibody (CAB3689) at 1:1000 dilution. THP-1 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Rig-I/DDX58 Monoclonal Antibody (CAB3689) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, generated using advanced technology, specifically targets the RIG-I/DDX58 protein in human samples and is validated for use in various applications, including Western blotting and immunofluorescence.The RIG-I/DDX58 protein is essential for antiviral immune responses, triggering signaling pathways that lead to the production of interferons and other cytokines to combat viral infections.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Rig-I/DDX58 Monoclonal Antibody
SKU:
CAB3689
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0824
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of the antiviral innate immune response. Mutations in this gene are associated with Singleton-Merten syndrome 2.
Purification Method
Affinity purification
Gene ID
23586
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Rig-I/DDX58 Rabbit mAb (CAB3689) at 1:1000 dilution. THP-1 cells were treated with LPS (1 μg/ml) at 37℃ for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
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