The RNASE2 Antibody (CAB9949) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to RNase2, this antibody enables the detection and analysis of the enzyme in various cell types, making it ideal for research in molecular biology and biotechnology.RNase2 is known for its role in RNA metabolism and its potential implications in diseases such as cancer and inflammatory disorders.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
RNASE2 Antibody
SKU:
CAB9949
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EDN, RAF3, RNS2, RNASE2
Positive Sample:
K-562, HL-60, Mouse liver
Cellular Localization:
Cytoplasmic Granule, Lysosome.
Calculated MW:
18kDa
Observed MW:
26kDa
The protein encoded by this gene is a non-secretory ribonuclease that belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein antimicrobial activity against viruses.
Purification Method
Affinity purification
Gene ID
6036
RRID
AB_2772027
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RNASE2 Rabbit pAb (CAB9949) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of paraffin-embedded mouse spleen using RNASE2 Rabbit pAb (CAB9949) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
