Anti-RING1B/RNF2 Antibody (CAB5563)

Antibody Name:	Anti-RING1B/RNF2 Antibody
Antibody SKU:	CAB5563
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP ChIP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human RING1B/RING1B/RNF2 (NP_009143.1).

Application:	WB IHC IF IP ChIP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, Jurkat, U-937, SH-SY5Y, Mouse brain, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human RING1B/RING1B/RNF2 (NP_009143.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSQA VQTN GTQP LSKT WELS LYEL QRTP QEAI TDGL EIVV SPRS LHSE LMCP ICLD MLKN TMTT KECL HRFC ADCI ITAL RSGN KECP TCRK KLVS KRSL RPDP NFDA LISK IYPS RDEY EAHQ ERVL ARIN KHNN QQAL SHSI EEGL KIQA MNRL QRGK KQQI ENGS GAED NGDS SHCS NAST HSNQ EAGP SNKR TKTS DDSG LELD NNNA AMAI DPVM DGAS EIEL VFRP HPTL MEKD DSAQ TRYI KTSG NATV DHLS KYLA VRLA LEEL RSKG ESNQ MNLD TASE KQYT IYIA TASG QFTV LNGS FSLE LVSE KYWK VNKP MELY YAPT KEHK
Gene ID:	6045
Uniprot:	Q99496
Cellular Location:	Chromosome, Nucleus
Calculated MW:	29kDa/37kDa
Observed MW:	38kDa

Synonyms:

RNF2, BAP-1, BAP1, DING, HIPI3, RING1B, RING2

Background:

Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity.

UniProt Protein Function:	RNF2: E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin- protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity. Component of chromatin-associated Polycomb (PcG) complexes. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2/RING2, MBLR, L3MBTL2 and YAF2. Component of a PRC1-like complex. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with RYBP, HIP2 and TFCP2. Association to the chromosomal DNA is cell-cycle dependent. Component of repressive BCOR complex containing Polycomb group subcomplex at least composed of RYBP, PCGF1, BCOR and RING1. Interacts with Interacts with PCGF2, CBX4, CBX6, CBX7 and CBX8. Interacts with CBX2, BMI and PHC2. Interacts with RYBP, HIP2 and TFCP2.
UniProt Protein Details:	Protein type:Ubiquitin ligase; EC 6.3.2.-; EC 6.3.2.19; Ligase; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 1q25.3 Cellular Component: nucleoplasm; nucleus; PcG protein complex; ubiquitin ligase complex Molecular Function:chromatin binding; protein binding; ubiquitin-protein ligase activity; zinc ion binding Biological Process: negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; protein sumoylation
NCBI Summary:	Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity. [provided by RefSeq, Jul 2008]
UniProt Code:	Q99496
NCBI GenInfo Identifier:	62901044
NCBI Gene ID:	6045
NCBI Accession:	Q99496.1
UniProt Secondary Accession:	Q99496,Q5TEN1, Q5TEN2, B2RBS7, B3KRH1,
UniProt Related Accession:	Q99496
Molecular Weight:	29,258 Da
NCBI Full Name:	E3 ubiquitin-protein ligase RING2
NCBI Synonym Full Names:	ring finger protein 2
NCBI Official Symbol:	RNF2Â Â
NCBI Official Synonym Symbols:	BAP1; DING; BAP-1; HIPI3; RING2; RING1BÂ Â
NCBI Protein Information:	E3 ubiquitin-protein ligase RING2
UniProt Protein Name:	E3 ubiquitin-protein ligase RING2
UniProt Synonym Protein Names:	Huntingtin-interacting protein 2-interacting protein 3; HIP2-interacting protein 3; Protein DinG; RING finger protein 1B; RING1b; RING finger protein 2; RING finger protein BAP-1
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	RNF2Â Â
UniProt Entry Name:	RING2_HUMAN

	Western blot analysis of extracts of various cell lines, using RING1B/RNF2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat lung using RING1B/RNF2 antibody at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using RING1B/RNF2 antibody at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using RING1B/RNF2 antibody at dilution of 1:100 (40x lens).
	Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug RING1B/RNF2 antibody at a dilition of 1:1000.
	Chromatin immunoprecipitation analysis of extracts of HeLa cells, using RING1B/RNF2 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Antibodies

Anti-RING1B/RNF2 Antibody (CAB18076)[KO Validated]

Anti-RING1B/RNF2 Antibody (CAB3564)

RNF2 Antibody (PACO11871)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)