Anti-RNH1 Antibody (CAB4079)

Antibody Name:	Anti-RNH1 Antibody
Antibody SKU:	CAB4079
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 202-461 of human RNH1 (NP_002930.2).

Application:	WB IF
Recommended Dilution:	WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Raji, U-87MG, HT-29, HeLa, Mouse liver, Mouse kidney, Mouse lung, Rat brain, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 202-461 of human RNH1 (NP_002930.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EALK LESC GVTS DNCR DLCG IVAS KASL RELA LGSN KLGD VGMA ELCP GLLH PSSR LRTL WIWE CGIT AKGC GDLC RVLR AKES LKEL SLAG NELG DEGA RLLC ETLL EPGC QLES LWVK SCSF TAAC CSHF SSVL AQNR FLLE LQIS NNRL EDAG VREL CQGL GQPG SVLR VLWL ADCD VSDS SCSS LAAT LLAN HSLR ELDL SNNC LGDA GILQ LVES VRQP GCLL EQLV LYDI YWSE EMED RLQA LEKD KPSL RVIS
Gene ID:	6050
Uniprot:	P13489
Cellular Location:	Cytoplasm
Calculated MW:	49kDa
Observed MW:	43kDa

Synonyms:

RNH1, RAI, RNH

Background:

Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50, 000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM, Jul 2010]

UniProt Protein Function:	RNH1: Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play a role in redox homeostasis.
UniProt Protein Details:	Protein type:Inhibitor Chromosomal Location of Human Ortholog: 11p15.5 Cellular Component: angiogenin-PRI complex; cytoplasm Molecular Function:protein binding; ribonuclease inhibitor activity Biological Process: negative regulation of catalytic activity; mRNA catabolic process; regulation of angiogenesis
NCBI Summary:	Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM, Jul 2010]
UniProt Code:	P13489
NCBI GenInfo Identifier:	132573
NCBI Gene ID:	6050
NCBI Accession:	P13489.2
UniProt Secondary Accession:	P13489,Q8IZK8, Q96FD7, Q9BQ80, Q9UDK6,
UniProt Related Accession:	P13489
Molecular Weight:
NCBI Full Name:	Ribonuclease inhibitor
NCBI Synonym Full Names:	ribonuclease/angiogenin inhibitor 1
NCBI Official Symbol:	RNH1
NCBI Official Synonym Symbols:	RAI; RNH
NCBI Protein Information:	ribonuclease inhibitor; placental RNase inhibitor; placental ribonuclease inhibitor
UniProt Protein Name:	Ribonuclease inhibitor
UniProt Synonym Protein Names:	Placental ribonuclease inhibitor; Placental RNase inhibitor; Ribonuclease/angiogenin inhibitor 1; RAI
Protein Family:	Ribonuclease inhibitor
UniProt Gene Name:	RNH1
UniProt Entry Name:	RINI_HUMAN

	Western blot analysis of extracts of various cell lines, using RNH1 antibody (CAB4079) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of C6 cells using RNH1 Rabbit pAb (CAB4079) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using RNH1 Rabbit pAb (CAB4079) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using RNH1 Rabbit pAb (CAB4079) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
RNH1 Antibody (PACO11878)	Human Ribonuclease Inhibitor / RNH1 ELISA Kit
	Human RI (Ribonuclease Inhibitor) CLIA Kit (HUES00795)
	Human Ribonuclease inhibitor (RNH1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)